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146(16):5102-5114
After washing the membrane
[4] Wang Y
super clean table or biosafety cabinet are easy to be contaminated by the mycoplasma
Up to 15 kb
Proteinase K Powder（RNase-Free, DNase-Free） _ 10401ES DNA&RNA Extraction 146(16):5102-5114Proteinase K is a serine protease with wide cleavage activity, which can cleave the carboxyl terminal peptide bond of aliphatic and aromatic amino acids. Its relative molecular weight is about 29. 3 kDa. Proteinase K is widely used in the preparation of chromosomal DNA for pulse electrophoresis, Western blotting, and the removal of a nuclease from DNA and RNA preparation. Denaturant such as SDS (1%) can improve its activity. In addition, the common
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