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4 Inject the solution in step 2 into the gel making glass plate
10×FuniCut™ Buffer
Leu23-Ser230
the protein migrates to 57-67 kDa based on SDS-PAGE result
Components A and B: Store at 4°C
LZCap™ AG(3'Ma-Biotin) _ 10691ES Restriction Enzymes 4 Inject the solution inLZCapTM AG(3'Ma Biotin) is a Cap 1 analog with a Biotin label, which can be used as the capping agent for producing mRNAs in an one pot process. Through T7 polymerase, mRNA with 5' end Cap 1 structure was generated by co transcription using LZCapTM AG(3'Ma Biotin), NTPs, and template DNA. The capped mRNA could be directly translated and expressed in cells and in vivo. mRNA capped with LZCapTM AG(3'Ma Biotin) can be bound to streptavidin magnetic beads
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